Suggested plates for best data: Fluorescence-top read; Solid Black flat-bottom for Excitations above 350 nm;. Temperature Control: ambient from +4 to 45C. Microscopes and Microplate Instrumentation. . Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Three data types for FP read types are also being introduced with v4. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Table 1. The instrument will read the plate, then the relative fluorescence units (RFUs) will be displayed in the Plate section of SoftMax Pro Software. 00. The SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. BioTek Synergy 2. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax. 0) OD (500 to 1100) RFU (5. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. 1 New Reader Support 1. 0 software was used to handle data analysis and control each instrument. 020 OD 2. 00. 5°C at 37°C. 25 to 3. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. 2 only) SpectraMax M-series Microplate readers. Bioz Stars score: 86/100, based on 1 PubMed citations. Molecular Devices SpectraMax M2 Multimode Microplate Reader w/ Laptop & Software. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. . 4. Reader Documentation Version No. Analyze the data Step 1: After the microplate has been read, theFluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. Selecting your dsDNA. For a higher . A solution for low volume applications. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. begin reading microplates with seven mouse clicks. Plate chamber design of the SpectraMax category 1 microplate readers. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. Microplate Reader Shelf. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Microplate readers’ standard detection modes are. 0 out of 5 $ 14,499 $ 13,995-13%. $22,500. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. When reading optical density at wavelengths below 340 nm, special UVtransparent,. Plate Readers. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. FI assay. Flexibility to read 6- to 384-well plates. $ 2,499. 2. Bottom Monochromator: Fluorescein 2. , a positive control). Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Fully tested with validations and warranty. View online or download Molecular devices SpectraMax M3 User Manual. 0 out of 5 $ 9,995 $ 9,495. When a. 0 × 107) RLU 0. Explore how the Gemini EM Microplate Reader is a flexible spectrofluorometer capable of running many fluorescence-based assays using its dual. variety of. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. D 4/21/06 9:53:29 AM . 10. For example, microplates containing different samp les can be read using the same or The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 306 plate air cal fail,low light: 316 grating motor driver faulty. SpectraMax® Microplate Readers and SoftMax® Pro Software. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The calculated LOQ values were 0. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. CA$14,500. Tristar 3 Multimode Microplate Reader. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Figure 2-23: Bottle holder To install the bottle holder: 1. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. Compact and tunable absorbance microplate readers DATA SHEET. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Refurbished. 3: The cuvette chamber. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. 1. 00 USD. Basic "FL Plate Reader start up protocol" (print and bring along for the. Package performance tests. Application: For molecular biology testing. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. Add to Cart. BMG Labtech - THERMOstar. 0 out of 5 $ 9,995 $ 9,495. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. The instrument is located in room 2131 at 300 George Street. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. Typical settings forTo maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax ® M2 Multi-Mode Microplate Reader. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. Data fromSpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 2 only) SpectraMax M-series Microplate readers. Multimode Readers Comparison Chart. Spectramax Multi-Mode Microplate Reader Performance Specifications. was 1. Filters: Excitation and emission slides for 4 filters each. patent nos. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2m2e user guide SpectraMax M2e user guide cover 1 1 42106 9:53:28 AM SpectraMax M2 SpectraMax…Spectramax i3 Plate Reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Microplate Reader, Multimode. 306 plate air cal fail,low light. If a lab requires instruments to read ELISA plates, one to do fluorescence and one for luminescence, it will likely look for one instrument that can handle all these. Typical settings for SpectraMax readers with fluorescence detection mode are shown. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. olecular saka. Biotek Synergy 2 Plate Reader Multi-Mode; Related products. The OliGreen assay from Molecular Probes, when run on a Gemini XS or Gemini EM fluorescence microplate reader with SoftMax Pro software, is a quick, sensitive detection method for single-stranded DNA. (See Figure 7. 6 % of reading 4. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax iD3 reader detects fluorescence using monochromators only. D 59 A. A . A solution for low volume applications. ~ Bio-Tek ELx808 Microplate Reader $2,795. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. D 47 7. ~ Bio-Tek ELx808 Microplate Reader $2,795. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. M2. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Two important settings to obtain the best results in TRF assays. Additionally, all BMG LABTECH microplate readers offer the possibility to measure in very small volumes, down to 2 µl of the sample by using the LVis Plate. 1) Select the correct plate and the wells that you want to measure. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers are as Fluorescence Validation Test Plates provide validation of the system’s optical characteristics. Fluorescence measurements no longer require you to adjust the gain. The plate reader. Published Dec 16, 2020 | Updated Dec 16, 2020. Step 4: Use the Template Editor to assign wells to ‘Standards’ and ‘Unknowns’ groups (Figure 2). Molecular devices SpectraMax M5 Pdf User Manuals. The assay is carried out in a standard 96-well plate. Perkin Elmer Envision. Data were analyzed with SoftMax ® Pro Software and graphed using a 4-parameter curve fit. Microplate Readers Include the M3, M4, M5, and M5. Double-click the unknown device with the yellow warning icon. Used. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 3: The cuvette chamber. Automated live cell imaging – cell counting and confluence. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Figure 2. S. 0) OD (0. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 00. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. When displaced, changes in fluorescence can be read in an FP, FI, or TR-FRET depending on the assay format. 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Molecular devices SpectraMax M3 Pdf User Manuals. Third this recommendation. spr SpectraMax iD5 SpectraTest ABS2 v1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Other features and benefits include: Eliminate changing out filters. Measure more accurately. Fluorescence Microplate Readers 4. Biotek Synergy HT Microplate Reader Multi-Mode. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. as a fluorescence blank. 25 to 3. Temperature control range from Ambient +4º to 50ºC. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. Because. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Biotek FLx800 Fluorescence Microplate Reader. I am wondering if the type of 96-well plate matters. BMG LABTECH. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. The plate is set up for reading and images are acquired according to specified parameters. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. New Life Scientific backs all orders with a 90-day warranty. Important things to consider when purchasing a multimode microplate reader. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. SpectraMax Microplate Readers. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. These lab workhorses are used in drug discovery. 0 software was used to handle data analysis and control each instrument. For a higher degree of automation, the Automation. Microplate Reader Shelf. The systems have. When connecting for the first time to either the SpectraMax L reader or StakMax system. The SpectraMax microplate readers support a wide variety of assay categories including . Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. STAKMAX. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. 5). on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. TRF as Secondary Detection Mode: TRF is supported as a secondary detection mode by these readers: Gemini XS, EM, XPS, M2, & FlexStation. SpectraMax Spectrofluorometers. 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。I am interested in using a SpectraMax M2 Plate Reader for a spectrophotometric assay and am trying to collect the proper reagents and materials. 1. This reader's dual monochromators for. 5%in96-and384-wellmicroplates. The cuvette must be in place when you read the plate. At different time points, the plate was removed from the incubator and loaded to the plate reader for fluorescence reading (excitation 450 nm,. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. 312 gain calibration failed. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Conclusions. In addition, the SpectraMax iD5SpectraMax Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single Fig 2: Enzymatic response of synthetic compounds. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader , SpectraMax M5. Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Add to Cart. Single-Label Plate Reader: SpectraMax Plus 384 (Molecular Devices). VANTAstar™ Flexible Multi-mode Microplate Reader. Microplate Readers. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 10. Specialized microplate readers for luminescence detection are available for use in applications such as luciferase reporter assays, bioluminescence resonance energy transfer (BRET), and other luminescent assays. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader andMicroplate Readers. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Category 2 and 3 microplate readers use gradient heating as well. Get unrivalled sensitivity and reproducibility and elevate your microplate assay protocol with the Eppendorf Assay/Reader Plates. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax ® M2 Multi-Mode Microplate Reader. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. The SpectraMax M2 reader can be easily converted and optimized for very low-throughput to medium-high throughput analysis, faster, more accurate results and saving reagents. 0 out of 5 $ 2,999-20%. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). 1. Driver installation. Page 28 Chapter 5: Read Modes and Read Types 1. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Brand: MOLECULAR DEVICES. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. Calcein AM is a widely used live-cell marker. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. 4. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Figure 1. This can increase throughput and efficiency, thus saving time and space in the lab. Multimode Reader Comparison Chart. Download Application Note Plate reader settings for NanoOrange assay. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. The SpectraMax 190 reader can be integrated with Molecular . Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. Cells in each image are identified by SoftMax Pro Software and cell-by-cellfluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. When the user applies a set temperature of 37°C, the top is heated to 37. The following general steps are taken with our absorbance and fluorescence readers. A microplate reader asPython 3. High tolerance for contaminants. Align the two slots on the bottle holder with the two pegs on the rail. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. . Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. It does not work as expected. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Cells in each image are identified by SoftMax Pro Software and cell-by-cell fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. 316 grating motor driver faulty. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. LuminescencePhotometricPerformance. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices SpectraMax M2 Multimode Plate Reader. Plate Shaking. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The plate is set up for reading and images are acquired according to specified parameters. The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. • Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 0 out of 5 $ 7,999. . With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. On microplate readers,. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Type. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Standard features include a cuvette port,. 0. Endpoint, kinetic, spectrum. Microplate Readers. Absorbance Fluorescence and fluorescence polarization Luminescence. Microplate Capacity. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. Add to Cart. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® Assays. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. SpectraMax Quant dsDNA Assay Kits. duAl monochromAtors With SpectraMax M2/M2e. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein. 0 × 107) RLU 0. In the M2, M5 and Plus. Description 1. Protein assays. PC software (SkanIt software) Dimensions (D x W x H) 58 x 53 x 51 cm (23 x 21 x 20 in) Weight. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE Summary of Contents for Molecular Devices SpectraMax M2. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Step 2: Click SoftMax Pro’s Read button. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. A decrease in fluorescence with increasing staurosporine concentration was detected with the SpectraMax iD3 reader. Description 1. 1. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. 5,112,134 5,766,875. Microplate Readers. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Download PDF. The GloMax® line includes high-performance plate readers with a range of detection options. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. The pre-configured protocols can be selected for each assay and. Stage and carriage are homed and position is tested and verified. Plate reader settings for NanoOrange assay. luminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. . 8 foot) 4400-0002 Power cord, 1 meter (3. Perkin Elmer 1420-2560 Victor X Series Plate Reader. 209, but in 96-well plate was 0. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Incubation. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Molecular Devices SpectraMax M2 Microplate Reader. For SpectraMax L or LMax II, LMax II. Molecular Devices SpectraMax M2 ; Molecular Devices. Using a black. 3. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Molecular Devices SpectraMax M2 Multimode Plate Reader. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. Spectramax Multi-Mode Microplate Reader Performance Specifications. . 67. 9000-0756. The analysis capabilities of SoftMax Pro Software provide quantitation in an easy-to-read, user-customizable report format. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 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